Topics covered include computational approaches and techniques for searching sequence, structural and expression databases and its relationship with disease databases, alignment and comparison of sequences by using dynamic programming, gene structure prediction, search for restriction sites, secondary structure prediction, generation of recombinant vectors in silico, obtaining the protein sequence encoded, folding and protein structure prediction, prediction of functional and protein-binding domains, protein interactions prediction, evaluating the pathogenicity of disease variants, molecular evolution and phylogenetic sequences analysis. Different examples will be reviewed in different areas and students make use of computational biology tools for analysis.